Protein production cro are biological macromolecules that function as building blocks of cells and play a major role in various physiological processes. They can be found in cells as native proteins or recombinant proteins produced using genetically modified bacteria, yeasts, insect or mammalian cell systems (Fig. 1). The recombinant proteins can be used for a broad range of applications including structural biology, biophysical assay development and screening, cellular and molecular biology studies as well as drug discovery.
Cloning and expression of recombinant proteins is an essential step in any research project. Recombinant protein production is a complex process and it is important to choose the correct system for your specific protein. Different expression systems have different advantages and disadvantages; the choice depends on the properties of the host system to clone the desired gene, the capacity of the protein to be folded into a functional conformation, the post-translational modifications required for full activity (such as glycosylation, phosphorylation, disulfide bond formation) and the intended downstream application.
Exploring Protein Production CROs: The Key Players in Biotechnology
Bacterial systems, such as E. coli, are the most commonly used expression systems for recombinant proteins. However, a number of recombinant proteins cannot be properly expressed in bacteria. Many of these proteins are unable to fold correctly and form insoluble aggregates (known as inclusion bodies). Others are not fully active because of the lack of certain posttranslational modifications or refolding conditions.
GenScript offers a wide range of recombinant protein expression services using genetically modified CHO and HEK293 cells. Our experience and expertise in protein expression allows us to provide our customers with high-quality recombinant proteins suitable for a variety of applications, including but not limited to structural biology and protein crystallography.
…